Zamkatimu
Pali njira zambiri zopezera bowa mycelium, ndipo zambiri zatsimikiziridwa pang'ono pang'ono pazaka zoyeserera mozama. Koma palinso njira zokonzekera mycelium, zomwe sizinali zangwiro ndipo zimafuna kufufuza kwina. Izi ndi zomwe akatswiri a mycologists amachita mu labotale komanso olima bowa osaphunzira omwe amalima mycelium ndi manja awo kunyumba.
M'chilengedwe, bowa nthawi zambiri amaberekana ndi spores, koma izi zitha kuchitikanso pogwiritsa ntchito zidutswa za bowa, zomwe alimi a bowa adazikhazikitsa kwa nthawi yayitali pogwiritsa ntchito mycelium wakuthengo ngati zobzala.
Momwe mungapangire mycelium kunyumba ikufotokozedwa mwatsatanetsatane patsamba lino.
Momwe anthu amalima okha mycelium
Poyamba, pofuna kukulitsa mitundu ina ya bowa, mwachitsanzo, champignons, anthu ankayang'ana ndowe ndikutenga mycelium kuchokera kumeneko. Ngati nyengo inali yovuta, ndipo panalibe mycelium mu zotayirako, ndiye kuti zimafalitsidwa mu greenhouses zapadera zowunikira. Pachifukwa ichi, dothi la manyowa (gawo lapansi) linakonzedwa ndipo mycelium inabzalidwa pamenepo, osaidzaza ndi nthaka, kotero kuti fruiting sanachitike. Atatha kuyembekezera kumera kwathunthu kwa mycelium mu gawo lapansi, olima bowa adatulutsa mycelium ndikuigwiritsa ntchito ngati zobzala. Zakudya zouma pang'ono zotere zitha kusungidwa kwa nthawi yayitali.
M'dziko Lathu, zinthu zobzala champignon zidapezekanso chimodzimodzi m'ma 30s. M'zaka za zana la XNUMX Komabe, pokula mycelium pogwiritsa ntchito njirayi, zokolola zinali zosauka, mycelium imachepa msanga, ndipo panthawi yobzala, tizilombo tachilendo tinkayambitsidwa, zomwe zimalepheretsa kukula kwa bowa ndikuchepetsa zipatso, choncho asayansi anapitirizabe kufufuza. njira zatsopano zolima.
Kumapeto kwa XIX atumwi. ku France, adakwanitsa kupanga chikhalidwe cha bowa wosabala champignon chomwe chimabzalidwa muzakudya zapadera kuchokera ku spores. Pokonzekera mycelium m'malo oyera, kuthekera kwa mycelium kudakula kwambiri, idakhazikika mwachangu, idakula kwambiri m'malo opatsa thanzi ndipo idabala zipatso kale kwambiri kuposa kugwiritsa ntchito hyphae "yakuthengo".
Kuyambira m'ma 20s. Ma laboratories a m'zaka za m'ma 30 ankagwira ntchito m'mayiko ambiri opanga bowa, samangodziwa kukonzekera mycelium, komanso momwe angapezere zipatso zabwino kwambiri. M’zaka za m’ma 1932. ku USSR, kuwonjezera pa kupeza mycelium pa kompositi wosawilitsidwa, zofalitsa zina zopatsa thanzi zidafunidwanso mwachangu. Mu XNUMX, njira yolima mycelium pambewu ya tirigu inali yovomerezeka. Pakalipano, alimi ambiri a bowa padziko lonse lapansi akugwira ntchito yolima mbewu za mycelium.
Kuipa kwa kukula mycelium mbewu
Monga momwe zimasonyezera, kuti mupeze mycelium, mbewu za mapira, balere, oats, tirigu, chimanga, rye ndi mbewu zina zimagwiritsidwa ntchito nthawi zambiri. Mukaweta bowa wa oyster ndi mbewu zina zomwe zimamera m'chilengedwe pamitengo, mycelium yofesa imakonzedwa pambewu, mankhusu a mpendadzuwa, pomace mphesa, utuchi, ndi zina zambiri.
Kutengera mtundu wa michere yomwe mycelium imamera, pali tirigu, gawo lapansi, mycelium yamadzi, ndi zina zambiri.
Mitundu yonse ya mycelium ikuwonetsedwa pachithunzichi:
Madzi a mycelium sakhala wamba, gawo lapansi la mycelium limagwiritsidwa ntchito pafupipafupi, koma chimanga chimagwiritsidwa ntchito kwambiri. Chifukwa chakuti mycelium ya njere, chifukwa cha zakudya zambewu, imathandizira kukula kwa mycelium, imagwiritsidwa ntchito polima bowa m'mafakitale.
Komabe, kukonzekera mycelium m'mafakitale kapena m'nyumba kumakhala ndi zovuta zake. Choyamba, izi ndi zofunika kuchuluka kwa khalidwe la mbewu yotseketsa. Ngati njirayi sinapambane, ndiye kuti nkhungu idzawoneka, kuteteza kukula kwa mycelium, zomwe zidzakhudzanso kukula kwa mbewu.
Moyo waufupi wa alumali wambewu mycelium (miyezi 2-3) ndizovuta kwambiri. Kuphatikiza apo, iyenera kusungidwa mufiriji pa kutentha kwa + 2-5 ° C, chifukwa izi zimachepetsa kukula kwa mycelium. Ngati kutentha kuli kwakukulu, izi zidzatsogolera kuti kukula kwa mycelium kupitirire, chifukwa chake idzadya mwamsanga chakudya ndi kufa.
Ndi maonekedwe a mycelium, n'zosatheka kudziwa tsiku la kupanga kwake. Chinthu chokha chomwe chingalangizidwe pankhaniyi ndi kukhala tcheru pogula pambali, chifukwa malo osungira sakanatha kuwonedwa. Wolima bowa wa novice apeza kuti mycelium ndi yotsika pakapita miyezi ingapo, pomwe sikudzakhala kwachabe kudikirira kukolola.
Choyipacho chingakhalenso chifukwa chakuti mycelium, wozoloŵera mbewu, "sadzafuna" kusamukira ku nkhuni.
Ndi gawo lapansi mycelium, zinthu ndi zosiyana, ndipo kuipa kwake kokha kumaonedwa kuti ndi kukula pang'onopang'ono, koma pali zowonjezera zambiri: sterility, kutha kusunga kutentha kwa chaka chimodzi.
Olima bowa amateur amakonda kukonda gawo lapansi la mycelium polima bowa pamitengo, popeza kufulumira kumera kulibe kanthu apa. Izi zimapitirira kwa miyezi ingapo chifukwa cha kuchulukana kwakukulu kwa mtengowo.
Ndikofunika kudziwa kuti mycelium yamtundu uliwonse imafa ngati itenthedwa kuposa 30 ° C.
Mabungwe onse akugwira ntchito yopanga mycelium, pomwe mikhalidwe yabwino kwambiri yolima imapangidwa. Ena amapeza mycelium kunyumba ndi chiyembekezo chopeza ndalama. Khalidwe lake silimakwaniritsa zofunikira nthawi zonse, koma, mwachilungamo, ndizoyenera kudziwa kuti nthawi zina pali akatswiri abwino kwambiri.
Bowa amatha kufalitsidwa ndi spores, koma kufalitsa kwa mycelium ndikwabwino kwambiri kwa wolima bowa woyambira, chifukwa kumapereka mwayi wopambana.
Kupitilira apo, njira yopezera mycelium imaganiziridwa mwatsatanetsatane, chifukwa nthawi zina zimangofunika kuzikulitsa nokha, mwachitsanzo, ngati pazifukwa zina mycelium yomwe imapezeka mwachilengedwe (mwachitsanzo, nkhuni kapena dothi lolowera ndi mycelium) osakwanira.
Mfundo zazikuluzikulu pokonzekera bowa mycelium ndi manja anu ndi awa. Choyamba, chidutswa chosabala cha minofu ya mafangasi chimachotsedwa ndikusamutsidwa kumalo opatsa thanzi (izi zimachitika m'magawo angapo, zomwe zidzakambidwe pansipa). Kenaka, zitsanzo zingapo zimapangidwa kuchokera ku chikhalidwe chachikulu, ndipo chisamaliro chiyenera kutengedwa makamaka kupewa matenda a chikhalidwe. Kenako, pangani malo ndi mikhalidwe yomwe imathandizira kwambiri kubereka kwa bowa.
Pochita izi, chikhalidwe chimasintha zotsatirazi: chikhalidwe chosabala pa agar sing'anga, chikhalidwe chosabala pambewu (grain mycelium) ndipo, pomaliza, kubala zipatso m'njira yopatsa thanzi.
Mawu oti "sterility" akhoza kukhala owopsa kwa oyamba kumene, koma ndikofunikira kuteteza chikhalidwe cha bowa wanu kuzinthu zambiri zowononga zomwe zimapezeka m'chilengedwe, ngakhale chilengedwe chili choyera chotani. Ndikofunika kwambiri kuwaletsa kuti asalowe mu chikhalidwe cholimidwa, chifukwa mwinamwake padzakhala "kulimbana" kwa zakudya zopatsa thanzi, ndipo chikhalidwe cha bowa chokha chiyenera kuzigwiritsa ntchito.
Ndi kulondola kwina komanso chizolowezi pochita njira zosavuta, njira yotsekera imatha kuchitidwa ndi aliyense.
Zotsatirazi zikufotokoza momwe mungakonzekere bowa mycelium agar.
Momwe mungapezere agar kwa mycelium kunyumba
Musanayambe kukonzekera mycelium kunyumba, muyenera kukonzekera sing'anga mchere agar. Agar wopangidwa kuchokera ku udzu wam'nyanja, pamodzi ndi zina zowonjezera, nthawi zambiri amagwiritsidwa ntchito kulima koyambirira komanso kudzipatula kwa chikhalidwe cha mafangasi.
Akatswiri amawonjezera zakudya zosiyanasiyana ku agar, mwachitsanzo, mchere, mankhwala opha tizilombo, etc. kuwathetsa pa magawo oyambirira kulima.
Monga momwe zimasonyezera, mutha kupanga mycelium nokha mumitundu yosiyanasiyana ya agar media. Zomwe zimagwiritsidwa ntchito kwambiri ndi mbatata ndi malto-dextrin agar. Mutha kuzipanga nokha kapena kugula zosakaniza zopangidwa ndi mafakitale m'sitolo.
Mukamagula agar m'sitolo, muyenera kugwiritsa ntchito ndalama zambiri, koma ndalama zowonjezera zimachotsedwa mosavuta, ndipo ngati muli ndi ndalama komanso mulibe nthawi yaulere, zosakaniza zopangidwa mwakonzeka zidzakhala zabwino kwambiri.
Ngati mumakonda kuchita zonse ndi manja anu, ndiye, malinga ndi akatswiri, mbatata agar kwa bowa mycelium kunyumba akhoza kukonzekera m'njira ziwiri. Njira zonsezi zimasiyana pang'ono. Kuonjezera apo, mutadziwana nawo, wolima bowa aliyense akhoza kubwera ndi njira yakeyake.
Mulimonsemo, kuti mupange mycelium ya bowa monga momwe teknoloji yoyenera ikusonyezera, muyenera kukonzekera: makapu oyezera, bandeji ya thonje, zojambulazo za aluminiyamu, chophikira chophikira, machubu oyesera okhala ndi zipewa zopangira autoclaving (akhoza kupezeka m'masitolo a zida zamankhwala) , ndodo yaying'ono yodzaza machubu oyesera , 2 mabotolo ndi voliyumu ya 1 l, ma flasks okhala ndi khosi lopapatiza.
Kenako, muphunzira kupanga mbatata mycelium agar mu njira yoyamba.
Njira yoyamba kukonzekera mbatata agar
Chiyerekezo cha zokolola za mankhwalawa ndi 1 lita.
Zosakaniza: Mbatata 300 g, 20 g agar (omwe akupezeka kumalo osungiramo zinthu zachipatala zoyenera, malo ogulitsa zakudya, kapena misika yaku Asia), 10 g dextrose kapena shuga wina, yisiti ya 2 g (akhoza kuperekedwa)
Njira ya ntchito.
Khwerero 1. Musanayambe kupanga agar kwa mycelium wa akhakula, muyenera kuwira mbatata ndi madzi okwanira 1 litre kwa ola limodzi. Kenako chotsani mbatata, kusiya msuzi wokha.
Khwerero 2. Msuzi, agar, shuga ndi yisiti (ngati mumagwiritsa ntchito) sakanizani bwino, mwachitsanzo, pogwiritsa ntchito whisk kuti mukwapule, simungathe kumenyana ndi kusakaniza uku.
Khwerero 3. Thirani zosakanizazo mu mabotolo kapena mabotolo ndi theka kapena magawo atatu mwa magawo atatu a voliyumu yawo.
Tsekani makosi ndi thonje swabs ndi kukulunga ndi zotayidwa zojambulazo. Thirani madzi mu chophikira chokakamiza kuti wosanjikiza wake kuchokera pansi pa mbale ukhale 150 mm, ndikuyika gululi kuti muyikemo mabotolo kapena mabotolo. Phimbani mbaleyo ndi chivindikiro ndikudula zingwe.
Khwerero 4. Ikani steamer pamoto ndikudikirira mpaka nthunzi ituluke. Pambuyo pa mpweya wabwino kwa mphindi zingapo (malingana ndi chitsanzo chenichenicho komanso motsatira malangizo), kutseka valve. Mabotolo amawiritsidwa pa 121 ° C (1 atm.) kwa mphindi 15. Panthawi imodzimodziyo, ndikofunikira kuonetsetsa kuti kutentha sikudutsa mlingo uwu, chifukwa pamenepa, caramelization ya sing'anga idzachitika, yomwe idzawonongeratu.
Khwerero 5. Pambuyo pa mphindi 15, zimitsani chitofu ndikusiya mbale kuti zizizire (pafupifupi mphindi 45). Kenako, osataya nthawi, tengani machubu oyesera aulere, chotsani zipewa ndikuyika zotengerazo pa tripod kapena m'zitini zoyera, kenako ndikuziyika pamalo oyeretsedwa kale ndi fumbi ndi dothi.
Khwerero 6. Mabotolo a sing'anga akazirala, achotseni pachophika chopopera pogwiritsa ntchito thaulo kapena mittens yakukhitchini. Sakanizani mopepuka, chotsani zojambulazo ndi swabs, pogwiritsa ntchito funnel, kutsanulira zomwe zili mu machubu oyesera pafupifupi gawo limodzi mwa magawo atatu.
Khwerero 7. Tsekani machubu oyesera ndi zipewa, koma mocheperapo kuposa kale, ikani mu chophikira chokakamiza, kuthira madzi ochulukirapo ngati kuli kofunikira. Mukafika kutentha kwa 121 ° C, siyani mbale pamoto kwa mphindi 30, ndikuzisiya kuti zizizirenso pang'onopang'ono mpaka kupanikizika kukufika pamlingo wabwinobwino.
Khwerero 8. Chotsani machubu ndikupukuta zisoti molimba. Konzani machubu pamalo opendekera. Chotsatira chake, pamwamba pa sing'anga ya agar iyenera kukhala pamtunda wokhudzana ndi botolo, motero kupanga malo ochuluka momwe angathere kuti mycelium ipangidwe (machubu oterowo nthawi zina amatchedwa "slant agar").
Sing'anga ikazizira, imakhala yofanana ndi odzola mosasinthasintha ndipo pamapeto pake imalimba kwambiri kotero kuti machubu amatha kuyikidwa molunjika ndipo sing'anga ya agar imakhalabe pamalo ake oyamba.
Vidiyo iyi ikufotokoza za kukonzekera kwa mycelium agar:
Machubu amatha kugwiritsidwa ntchito nthawi yomweyo kapena pakatha milungu kapena miyezi. Pamapeto pake, ayenera kuikidwa mufiriji, ndipo musanagwiritse ntchito, onetsetsani kuti palibe zizindikiro za nkhungu kapena kuipitsidwa ndi mabakiteriya pa sing'anga.
Gawo lotsatira la nkhaniyi likukhudzana ndi momwe mungapezere mbatata ya mycelium agar kunyumba mwanjira ina.
Momwe mungapangire agar kwa mycelium kunyumba mwanjira ina
Chiyerekezo cha zokolola za mankhwalawa ndi 1 lita.
Zosakaniza:
- 284 g mbatata,
- 21,3 g (3/4 oz) agar
- 8 g dextrose (mutha kugwiritsa ntchito shuga wa tebulo m'malo mwake).
Njira ya ntchito.
Khwerero 1. Kuti mupange agar wa mycelium ndi manja anu, muyenera kutsuka mbatata ndikuzidula mu zidutswa zing'onozing'ono, kusiya zikopa, ndikuziwiritsa mu 0,5 malita a madzi mpaka mutaphika. Chotsani mbatata ndi nyenyeswa zawo. Thirani madzi okwanira 1 litre mu mbale yachitsulo kapena galasi ndikuwonjezera dextrose (shuga), decoction ndi agar kwa izo.
Khwerero 2. Sungunulani agar. Kuti muchite izi, ikani chosakaniza cha agar mu mbale yophimbidwa ndi zojambulazo za aluminiyamu mu chophikira chokakamiza. Yatsani chophikira chokakamiza ku 121 ° C (1 atm) ndikuchoka. Pambuyo pa mphindi 20, agar adzasungunuka kwathunthu. Kenako zimitsani chitofucho ndikusiya chophikira chokakamiza kuti chizizire pang'onopang'ono.
Khwerero 3. Pogwiritsa ntchito mittens kapena matawulo akukhitchini, tsanulirani kusakaniza ndi agar wosungunuka mu machubu oyesera (kapena mabotolo ang'onoang'ono) mpaka gawo limodzi mwa magawo atatu a voliyumu. Ikani machubu oyesera pachoyikapo kapena muzitini. Thirani zotsalira za agar mu botolo, kutseka ndi thonje kapena padding tampon ndi samatenthetsa pambuyo pake, pamodzi ndi machubu ena onse oyesera.
Makapu a machubu oyesera kapena zivindikiro sayenera kutsekedwa mwamphamvu. Pankhaniyi, kupanikizika kudzakhala kofanana panthawi yotseketsa. Ngati ma tamponi a thonje kapena padding agwiritsidwa ntchito potseka, simungasamale za kufananiza kwamphamvu, komabe, machubu oyeserera amayenera kuphimbidwa ndi zojambulazo za aluminiyamu, apo ayi condensate ya chophikira chozizira imagwera pazoyimitsa.
Khwerero 4. Samalani agar, omwe machubu oyesera (mabotolo) ayenera kuikidwa mu chophikira chokakamiza ndikusungidwa pa kutentha kwa 121 ° C (1 atm.) kwa mphindi 25, osaphatikiza nthawi yomwe yagwiritsidwa ntchito kuti mukwaniritse kukakamizidwa kofunikira. Kenako zimitsani chitofucho ndikusiya mbale zizizire pang’onopang’ono. Kutsika kofulumira kwa kupanikizika sikuyenera kuloledwa, chifukwa izi zingayambitse agar mu machubu kuwira, kuphulika kupyolera mu swabs ndi zipewa zoyimitsa, zomwe zingayambitse matenda.
Khwerero 5. Pamapeto pake, kusakaniza mu machubu oyesera kumapeza malo okonda. Kuti muchite izi, pukutani pamwamba pomwe machubu oyesera adzayikidwa ndi 10% bleach solution yokhala ndi chlorine. Pasakhale zojambula m'chipindamo.
Mothandizidwa ndi mittens yakukhitchini kapena chopukutira chophika chopondera, chotsani machubu oyesera otentha ndikuyika patebulo molunjika, ndikutsamira chidebecho ndi mbali imodzi motsutsana ndi chinthu china. Izi zisanachitike, ndikofunikira kusankha njira yoyenera yogwiritsira ntchito zinthu zakunja (mipiringidzo, mulu wa magazini, ndi zina).
Pamene agar ayamba kulimba, kusandulika kukhala odzola, kutseka zisoti (mapulagi) mu machubu oyesera mwamphamvu kwambiri.
Sungani mbatata ya agar m'machubu oyesera pamalo ozizira, opanda fumbi.
Onerani kanema wamomwe mungapangire agar kwa mycelium ndi manja anu:
Gawo lomaliza la nkhaniyi likukhudzana ndi momwe mungakulire bwino bowa mycelium.
Kodi kuphika bowa mycelium kunyumba
Musanakulire mycelium kunyumba, konzani: scalpel (mpeni wakuthwa wokhala ndi tsamba lopyapyala), nyali yamzimu (nyali ya propane yokhala ndi canister, chowunikira kapena machesi), zitini zachitsulo kapena zoyikapo zoyesa machubu okhala ndi agar wokhazikika komanso mayeso okonzekera. machubu, chofukizira scalpel kapena mpeni, bandeji ya microporous (bandeji yokhazikika bwino), botolo lopopera losakaniza ndi 1 gawo la bulichi ndi chlorine ndi magawo 9 a madzi (ngati mukufuna), thupi loyera la bowa (ngati ndinu woyamba, ndiye bwino kugwiritsa ntchito bowa wa oyisitara).
Njira ya ntchito.
Khwerero 1. Musanakulire mycelium, muyenera kukonzekera malo okhazikika (tebulo, kauntala) ndikutsuka ndi madzi otentha a sopo ndikupukuta. Kuti mupereke mankhwala ophera tizilombo, tsitsani pamwamba ndi aerosol ndi 10% bleach solution, pukutani bwino ndi chiguduli choyera kapena thaulo lamapepala. Tsekani mazenera kuti mupewe kufalikira kwa mpweya momwe mungathere. Ndi bwino kugwira ntchito m'mawa, pamene mlengalenga muli fumbi pang'ono.
Khwerero 2. Kuti mukule mycelium kunyumba, muyenera kukonza malo ogwirira ntchito: ikani zida ndi zida zomwe zingatheke komanso mwadongosolo, zokonzekera ntchito.
Tengani machubu a agar ndikuyika mu zitini zachitsulo kapena pazitsulo. Yatsani kuwala ndi mosamala samatenthetsa tsamba la mpeni (scalpel) pamoto, anachiyika pa choyimira, mwachitsanzo, zopangidwa ndi waya. Choyimiliracho chikufunika kuti mpeni ukhale pafupi ndi moto pamene chida sichikugwiritsidwa ntchito.
Khwerero 3. Tengani bowa mwatsopano woyera. Ngakhale kuti kunja kwake kumakhala ndi tizilombo toyambitsa matenda ndi nkhungu zambiri, nthawi zambiri mulibe zamoyo zamkati zomwe zingayambitse matenda, ndithudi, ngati mulibe madzi ambiri mu bowa.
N'zosatheka kuthyola mbali ya bowa, chifukwa tsambalo limalowa mkati mwa bowa ndi mabakiteriya kuchokera kunja. Ikani bowa patebulo ndi malo odetsedwa (woyera sayenera kukumana ndi tebulo).
Chofunikira ndichakuti muyenera kupanga malo oyera otseguka, kenaka mutenge kachidutswa kakang'ono ka bowa, kamene kamayikidwa mu chubu choyesera.
Khwerero 4. Kuti mukule mycelium nokha, konzani zida ndi zida m'njira yoti chubu choyesera chitsegulidwe pang'ono musanadzaze ndi minofu ya bowa. Kuchepetsa mwayi wa matenda, chubu choyesera (kapena choyimitsa, kapu) sichiyenera kuikidwa pamalo ogwirira ntchito, omwe ndi ovuta kwambiri, choncho ndizomveka kuchita ndi chubu choyesera chopanda kanthu pasadakhale.
Khwerero 5. Kutsatizana kwina kumatsimikiziridwa makamaka ngati munthu wamanja kapena wamanzere akugwira ntchitoyi, zochita za munthu wamanja zikufotokozedwa pansipa.
Chala chachikulu chakumanzere chili pansi, pamene china chili chopingasa. Ikani chubu choyesera pakati pa zala zapakati ndi mphete. Pankhaniyi, chala cha mphete chili pamwamba, chala chapakati chili pansi pa botolo, ndipo cork (chivundikiro) chimayendetsedwa kutali ndi dzanja. Sikoyenera kupendekera chubu choyesera, malo opingasa okha amafunikira pano, apo ayi tinthu tating'onoting'ono towuluka mlengalenga tidzakhala ndi mwayi waukulu wolowera m'khosi mwa chidebecho. Mayendedwe a chubu ndi kotero kuti bevelled pamwamba pa agar amalunjika mmwamba. Ndiko komwe minofu ya bowa idzabzalidwe.
Khwerero 6. Chotsani mosamala choyimitsa (chivundikiro) kuchokera pa chubu choyesera ndikutenga chomalizacho m'njira yosonyezedwa.
Pogwiritsa ntchito chala chaulere ndi chala chachikulu chakumanzere, tengani chidutswa cha bowa chokhala ndi malo oyera. Ndi dzanja lanu lamanja, tenga msanga chowolerapo ngati pensulo kapena cholembera. Pogwiritsa ntchito nsonga ya tsamba, sungani mosamala kachidutswa kakang'ono ka bowa wa triangular kuchokera ku minofu yoyera ya bowa ndipo, nthawi yomweyo, kuika mu botolo m'mphepete mwa khosi, ngati kuli kofunikira, ndikugwedeza nsonga ya scalpel ndikugogoda. mayendedwe. Ikani scalpel mmbuyo ndipo mwamsanga mutseke chubu ndi choyimitsa.
Khwerero 7. Dinani chubu pang'ono m'manja mwanu kuti musunthire chidutswa cha bowa pamwamba pa agar. Ikani chubucho mu malata ena osungiramo machubu omwe amalowamo.
Ngati malingalirowo akutsatiridwa ndendende, pali mwayi woti chikhalidwe cha bowa chobzalidwa chinali choyera.
Zotsatira zofananazo zimachitidwa ndi ma flasks ena ndi zinthu za bowa. Ndikofunika kukonzekera machubu angapo oyesera kuchokera ku bowa limodzi, chifukwa ziribe kanthu momwe ntchitoyo imagwirira ntchito mosamala komanso mwaukhondo, matenda amapezeka nthawi zambiri.
Bowa litalowetsedwa mu chubu choyesera (njirayi imatchedwa inoculation), scalpel iyenera kutsekedwanso pamoto.
Mukamaliza ndi machubu oyesera, muyenera kutseka choyimitsa mwamphamvu momwe mungathere ndikukulunga malowo ndi tepi ya microporous, yomwe singalepheretse bowa "kupuma" ndipo nthawi yomweyo sangalole kuti mabakiteriya alowe mu chubu choyesera. khosi.
Ndikoyenera kuyika zomata pa botolo lililonse kapena kulemba zolembera zomwe zikuwonetsa tsiku ndi zomwe zili mkati.
Machubu oyesera okonzeka amasungidwa m'malo amdima komanso ozizira pa kutentha koyenera kwa 13-21 °C. Patapita nthawi (masiku angapo kapena sabata), minofu ya bowa imadzaza ndi fluff, zomwe zimasonyeza kuyamba kwa mycelium. Pambuyo pa masabata angapo, mycelium idzasefukira pamwamba pa agar.
Pamaso pa nkhungu, yomwe imadziwika mosavuta ndi spores zobiriwira kapena zakuda, kapena kuipitsidwa ndi bakiteriya (monga lamulo, zikuwoneka ngati chinthu chonyezimira), zomwe zili mu chubu choyesera ziyenera kutayidwa nthawi yomweyo ndikutsukidwa ndi madzi otentha a sopo pamodzi. ndi cork. Ngati n'kotheka, machubu oyezera odwala amatsekeredwa m'chipinda china momwe mulibe zikhalidwe zathanzi.
Tsatanetsatane wa momwe mungakulire mycelium ikufotokozedwa muvidiyoyi:
